Next Generation Sequencing Facilitates Quantitative Analysis of Transcriptomes Caused by Dexamethasone Treatment in Cancer Cells

As commonly used drug in clinical, there is little known about the effects of dexamethasone (Dex) on cancer cells. The goals of this study are to explore the transcriptome caused by Dex in cancer cells. Seven cancer cell lines were used in this project, gastric cancer cell lines SGC-7901 and MKN-45, and colorectal cancer cell lines NCIH-747 and RKO, liver cancer cell line SMMC-7721, pancreatic cancer cell line BxPC3, melanoma cell line MV3. After treated by Dex for 48 hours, total RNAs were extracted for next-generation sequencing profiling. RNA profiles of seven cancer cell lines after DMSO or Dex treatment were generated by deep sequencing, using Illumina HiSeq 2000.