Data from: DNA extraction optimisation for minute land snails of the genus Vertigo O.F. Muller, 1773 (Gastropoda: Vertiginidae): a comparative evaluation of six methods including a non-destructive shell-preserving protocol

This dataset contains the summary data underlying all tables and figures in the manuscript "DNA extraction optimisation for minute land snails of the genus Vertigo O.F. Muller, 1773 (Gastropoda: Vertiginidae): a comparative evaluation of six methods including a non-destructive shell-preserving protocol" submitted to Molecular Ecology Resources. Six DNA extraction methods were compared using Vertigo antivertigo preserved in 96% ethanol (n = 10 per method). Metrics include DNA concentration (QuantiFluor dsDNA), spectrophotometric purity (NanoDrop A260/280 and A260/230), PCR amplification success across four loci (COI, COI mini, ITS1, ITS2), Bioanalyzer fragment analysis in the 200-1000 bp window, and cost-per-result estimates. The workbook contains README, Table1_Methods, Table2_Yield_Purity, Table3_PCR_Success, Table4_Cost, Table5_Bioanalyzer, and GenBank_Sequences sheets. Representative ITS1 sequences are deposited in GenBank under accessions PZ227160-PZ227162. Specimens were collected under permit No. 600000-S-55 (Lithuanian Environmental Protection Agency, 2020-06-08).