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Comparison of R-loop occurrence between wild-type and nusG mutant of Escherichia coli. Escherichia coli K-12

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The prevalence and positions of chromosomal R-loops were estimated in three E. coli strains, namely, wild-type, nusG mutant and nusG mutant with multicopy plasmid bearing gene for RNase HI, following exposure of non-denatured nucleic acid preparations from the strains to sodium bisulfite followed by whole-genome sequencing.

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2012-10-23
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