Table_8_Analysis of the Interaction Network of Hub miRNAs-Hub Genes, Being Involved in Idiopathic Pulmonary Fibers and Its Emerging Role in Non-small Cell Lung Cancer.DOCX

Idiopathic pulmonary fibrosis (IPF) is a fibrotic interstitial lung disease with lesions confined to the lungs. To identify meaningful microRNA (miRNA) and gene modules related to the IPF progression, GSE32537 (RNA-sequencing data) and GSE32538 (miRNA-sequencing data) were downloaded and processed, and then weighted gene co-expression network analysis (WGCNA) was applied to construct gene co-expression networks and miRNA co-expression networks. GSE10667, GSE70866, and GSE27430 were used to make a reasonable validation for the results and evaluate the clinical significance of the genes and the miRNAs. Six hub genes (COL3A1, COL1A2, OGN, COL15A1, ASPN, and MXRA5) and seven hub miRNAs (hsa-let-7b-5p, hsa-miR-26a-5p, hsa-miR-25-3p, hsa-miR-29c-3p, hsa-let-7c-5p, hsa-miR-29b-3p, and hsa-miR-26b-5p) were clarified and validated. Meanwhile, iteration network of hub miRNAs-hub genes was constructed, and the emerging role of the network being involved in non-small cell lung cancer (NSCLC) was also analyzed by several webtools. The expression levels of hub genes were different between normal lung tissues and NSCLC tissues. Six genes (COL3A1, COL1A2, OGN, COL15A1, ASPN, and MXRA5) and three miRNAs (hsa-miR-29c-3p, hsa-let-7c-5p, and hsa-miR-29b-3p) were related to the survival time of lung adenocarcinoma (LUAD). The interaction network of hub miRNAs-hub genes might provide common mechanisms involving in IPF and NSCLC. More importantly, useful clues were provided for clinical treatment of both diseases based on novel molecular advances.

原发性肺纤维化（IPF）是一种局限于肺部的纤维化间质性肺疾病。为识别与IPF进展相关的有意义的小分子RNA（miRNA）和基因模块，下载并处理了GSE32537（RNA测序数据）和GSE32538（miRNA测序数据），随后应用加权基因共表达网络分析（WGCNA）构建基因共表达网络和miRNA共表达网络。GSE10667、GSE70866和GSE27430被用于对结果进行合理的验证，并评估基因和miRNA的临床意义。确定了六个核心基因（COL3A1、COL1A2、OGN、COL15A1、ASPN和MXRA5）和七个核心miRNA（hsa-let-7b-5p、hsa-miR-26a-5p、hsa-miR-25-3p、hsa-miR-29c-3p、hsa-let-7c-5p、hsa-miR-29b-3p和hsa-miR-26b-5p），并对它们进行了明确和验证。同时，构建了核心miRNA-核心基因的迭代网络，并利用多个网络工具分析了该网络在非小细胞肺癌（NSCLC）中可能发挥的新兴作用。核心基因的表达水平在正常肺组织和NSCLC组织中存在差异。六个基因（COL3A1、COL1A2、OGN、COL15A1、ASPN和MXRA5）和三个miRNA（hsa-miR-29c-3p、hsa-let-7c-5p和hsa-miR-29b-3p）与肺腺癌（LUAD）的生存时间相关。核心miRNA-核心基因的相互作用网络可能揭示了涉及IPF和NSCLC的共同机制。更重要的是，基于新的分子进展，为这两种疾病的治疗提供了有价值的线索。