Time Course of Gene Expression in the Substantia Nigra in Response to Intrastriatal 6-hydroxydopamine in the rat.

Comprehensive gene expression analysis was conducted to detect significant differences in gene expression. Comparisons were conducted within the same treatment groups over time as well as across treatments within the same post-treatment interval. Longitudinal expression patterns were parsed using a k-means clustering algorithm as a method for finding patterns indicative of neuronal loss, upregulation in response to lesion or surgical damage exclusive of lesion.本研究开展全面的基因表达分析，旨在检测基因表达的显著差异。分析同时涵盖同一处理组内随时间推移的表达对比，以及相同治疗后时段内不同处理组间的表达对比。本研究采用k均值聚类算法（k-means clustering）解析纵向基因表达谱，以挖掘两类特征模式：一类可指示神经元丢失，另一类为响应于损伤（lesion）或手术损伤（排除损伤本身诱导）的表达上调。