Argonaute 2 associated miRNAs in CA1, CA3 and Dentate Gyrus hippocampal subfields from the rat perforant pathway stimulation (PPS) epilepsy model

MicroRNAs (miRNAs) are short noncoding RNAs that shape the gene expression landscape, including during the pathogenesis of temporal lobe epilepsy (TLE). In order to provide a full catalog of the miRNA changes that happen during experimental TLE, we sequenced Argonaute 2-loaded miRNAs in CA1, CA3 and Dentate Gyrus hippocampal subfields from the rat perforant pathway stimulation (PPS) model at regular intervals between the time of the initial precipitating insult to the establishment of spontaneous recurrent seizures.微小核糖核酸（MicroRNAs, miRNAs）是一类短链非编码RNA，可调控基因表达谱，在颞叶癫痫（temporal lobe epilepsy, TLE）的发病进程中发挥重要作用。为全面收录实验性颞叶癫痫发生过程中miRNA的表达变化情况，本研究对大鼠穿通通路刺激（perforant pathway stimulation, PPS）模型的海马CA1、CA3及齿状回亚区中装载于Argonaute 2的miRNA进行了测序，测序时间覆盖从初始致痫损伤到自发性复发性癫痫发作形成的多个固定时间间隔。