James R. LaFountain, Rudolf Oldenbourg (2011) CIL:12028, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. CIL. Dataset

Time series of meiosis I, starting at metaphase, in primary spermatocytes of the crane-fly Nephrotoma suturalis that have been experimentally flattened.
Time-lapse polarization microscopy using a Nikon
Microphot SA, equipped for liquid crystal polarized light microscopy
(LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil immersion condenser, with 2.0x zoom lens. Images captured every 15 sec over 35 min using a QImaging Retigo EXi CCD camera. Raw images were processed
using 5-frame algorithm (Shribak and Oldenbourg, 2003). A movie of the series is included in this grouped set.

本数据集记录了蜻蜓科昆虫Nephrotoma suturalis初级精母细胞减数第一次分裂（减数I期）的时序变化，起始点为中期。采用Nikon Microphot SA显微镜，配备液晶偏振光显微镜系统（LC-PolScope，CRi，Woburn，马萨诸塞州），搭载60x/1.4 PlanApo油浸物镜、1.4 NA油浸聚光镜及2.0倍放大镜头进行时间间隔摄影。利用QImaging Retigo EXi CCD相机，每15秒捕捉一次图像，持续35分钟。原始图像经过Shribak和Oldenbourg（2003）提出的5帧算法进行处理。该系列图像中包含一组时序视频。