Diversity, adaptation and metabolic potential of the microbiome in biofilms from a high-temperature hot spring

Hot spring microbiomes have garnered significant research attention from exploring the diversity of prokaryotic communities to genes and functional potentials. While cyanobacteria-rich biofilms, characterized by warm temperatures, have been extensively studied, there is limited investigation into high-temperature streamer biofilm communities (SBC) devoid of photosynthetic ability. Here, we studied the biofilm of a Dusun Tua (DT) hot spring with a temperature of 75°C and a pH of 7.6. This grey-tan colored biofilm appeared at sites where water had slowed down following the deposition of plants and inorganic debris along the hot spring after a flood event. Amplicon sequencing of V3-V4 regions of 16S rRNA showed that dominant phyla included the Aquificota, Chloroflexota, and Desulfobacterota together with other abundant amplicon sequence variants from the Bacteroidota, Deinococcota, Hydrothermae and Armatimonadota. These microbial populations appeared to be distinct from other reported SBCs..., Methodology
Sampling and DNA extraction
The Dusun Tua (DT) hot spring is located at 3.136838613811952, 101.83446401515266. Sampling trips were performed in July 2022 (labelled DT-7) and November 2022 (DT-11). Samples of the gray-tan biofilm were randomly collected and immediately transported to the laboratory and frozen at −20 °C within 12 h. The bulk genomes of the samples were extracted using SPINeasyTM DNA extraction kit (MP Biomedicals, Irvin, CA, USA) according to the manufacturer’s recommended protocol. DT biofilm samples were lysed via bead beating, following 3 cycles (20 Hz) for 1 minutes each. The extracted DNA were evaluated using a NanodropTM 1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA), a Qubit 3.0 Fluorometer (Invitrogen, Merelbeke, Belgium) and with a 1% w/v agarose gel electrophoresis.
 
Shotgun sequencing and data processing
The duplicated extracted metagenomic DNA from each sampling time were pooled prior to fragmentation by a Covaris sonicator (Covar..., , ```
# Diversity, adaptation and metabolic potential of the microbiome in biofilms from a high-temperature hot spring

https://doi:10.5061/dryad.sn02v6xcd

Raw reads for amplicon and shotgun sequencing data are publicly available in NCBI with BioProject number PRJNA1082868 and BioSample accessions SAMN40219291 and SAMN40219292. The contigs for medium- and high-quality metagenome-assembled genomes (MAGs) are available in this open-access repository Dryad platform. This dataset are the MAGs fasta files of a biofilm found in a hot spring.

## Description of the data and file structure

The MAGs information can be used as part of microbiology and bioinformatic analyses. This may include genome-to-genome comparison between individual MAG with closely related MAG or cultured microbial genomes. The fasta files can be used for phylogenetic tree construction. Besides, the files have ample of genes sequences suitable for gene mining.There is no missing data codes. The files are named from bin....