Seminal Plasma cell-free DNA

Seminal fluid is a complex mixture of secretions from the seminal gland, prostate, testes, epididymis, and bulbourethral glands. It not only serves as a medium for sperm transport but also provides nutrients and a protective environment for spermatozoa during their journey through the female reproductive tract. Seminal fluid contains a complex range of organic and inorganic constituents, including proteins, enzymes, lipids, carbohydrates, and various ions, which play crucial roles in sperm motility and viability. Despite the emerging interest in liquid biopsies in cancer diagnostics and beyond, seminal fluid has not been extensively studied as a potential specimen type. Here we characterize the composition of cell-free DNA (cfDNA) in seminal fluid, highlighting its potential use in the liquid biopsy space. This study characterizes seminal plasma cfDNA, including DNA methylation profiles of men with and without vasectomies.