Induction of extracellular adenosine salvage and metabolic quiescence regulate the transitional to follicular B cell checkpoint in humans.

Transcriptional profiling of transitional and follicular B cells showed that metabolic quiescence was acquired at the follicular B cell stage in both humans and mice, characterized by an orchestrated reduction of mTORC1 signaling, aerobic respiration and expression of genes involved in ribosome biogenesis. RNAseq analysis of transitional and follicular B cells from healthy human donors and C57BL/6 (wild-type) mice对过渡型B细胞（transitional B cells）与滤泡型B细胞（follicular B cells）的转录谱分析（transcriptional profiling）显示，人类与小鼠的代谢静息状态（metabolic quiescence）均于滤泡型B细胞阶段获得，其特征为mTORC1（mTOR complex 1）信号通路、有氧呼吸（aerobic respiration）以及核糖体生物发生（ribosome biogenesis）相关基因的表达呈现有序下调。针对健康人类供体及C57BL/6（野生型）小鼠的过渡型与滤泡型B细胞的RNA测序（RNA-seq）分析