The genetic basis of incipient sexual isolation in Drosophila melanogaster

Speciation is a fundamental evolutionary process, but the genetic changes accompanying speciation are difficult to determine since true species do not produce viable and fertile offspring. Partially reproductively isolated incipient species are useful for assessing genetic changes that occur prior to speciation. Drosophila melanogaster from Zimbabwe, Africa are partially sexually isolated from other D. melanogaster populations whose males have poor mating success with Zimbabwe females. We used the North American D. melanogaster Genetic Reference Panel (DGRP) to show that there is significant genetic variation in the mating success of DGRP males with Zimbabwe females, to map genetic variants and genes associated with variation in mating success, and to determine whether mating success to Zimbabwe females is associated with other quantitative traits previously measured in the DGRP. Incipient sexual isolation is highly polygenic and associated with the common African inversion In(3R)K and ..., , , # The genetic basis of incipient sexual isolation in *Drosophila melanogaster*

## Description of data and files

### adjustedData.RData

This RData contains the following objects:

* adjustPheno: R function to adjust phenotype
* inv: table for inversion genotypes where the row names are the line IDs and column names are the inversion names. In the table, \"0\" = reference karyotype; \"1\" = heterozygous for the inversion, \"2\" = homozygous for the inverted karyotype.
* pcs: top 11 PCs for the genotypes where the row names are the line IDs and column names are the PC names
* wolba: Wolbachia infection status where the row names are the line IDs and there is one column with \"y\" indicating infected lines and \"n\" indicating Wolbachia free.
* writeCovar: returns a matrix of covariates, which can be used for other programs

### Z30mating.csv

Z30 mating raw data, comma-separated file with the following columns:

* series: series is similar to blocks where assays are performed in the s...