Single Cell RNA-Sequencing in Adenoid Cystic Carcinoma

This study of salivary adenoid cystic carcinoma aimed to profile individual malignant and stromal cells in treatment-naive head and neck adenoid cystic carcinoma, with or without metastatic cervical lymph nodes. Clinical and pathologic information was collected on all patients, in addition to a fresh biopsy sample from the primary tumor and/or metastatic lymph node at the time of initial surgery. A fresh biopsy sample from a matched local recurrence was also collected for a single unique patient. Biopsy specimens were processed for single cell RNA-seq. A total of ~1,700 single cells from seven participants were included in the final dataset.]]>
Participants with primary treatment-naive histologically confirmed adenoid cystic carcinoma of the head and neck were included. Participants were excluded if pathology did not conclusively demonstrate adenoid cystic carcinoma.]]>
The first primary tumor sample included in the study was collected in April 2016. The last sample was collected in June 2018. Sequencing data were generated and analyzed by June 2020.]]>