Transcription profiling of mouse wildtype and alb/cre liver-conditional Pdss2 knockouts

Utilizing M. musculus as a model of mitochondrial dysfunction provides insight into cellular adaptations which occur as a consequence of genetic alterations causative of human disease. We characterized genome-wide expression profiles of liver-conditional knockout mice for Pdss2 compared with loxP controls. Our goal was to detect concordant changes among clusters of genes that comprise defined metabolic pathways utilizing gene set enrichment analysis. Experiment Overall Design: Liver from three biological replicates each of wildtype and coenzyme Q biosynthetic mutant M. musculus were used as sources of total RNA for hybridization to Affymetrix whole-genome microarrays. Comparison of the data was intended to reveal metabolic pathway alterations downstream of the mutation.以小家鼠（Mus musculus）作为线粒体功能障碍的研究模型，可揭示由导致人类疾病的遗传改变所引发的细胞适应性变化。本研究对Pdss2基因肝脏条件性敲除小鼠与loxP对照小鼠的全基因组表达谱进行了表征。本研究的目标是借助基因集富集分析（gene set enrichment analysis），在构成特定代谢通路的基因簇中检测一致性的表达变化。 实验总体设计：分别采集3份生物学重复的野生型与辅酶Q生物合成突变型小家鼠的肝脏组织作为总RNA提取样本，用于与Affymetrix全基因组芯片进行杂交。本研究通过数据比较分析，旨在揭示该突变下游的代谢通路改变。