Quantitative Transcriptomes Analysis of Wild Type and ompR mutant of K. pneumoniae ATCC43816 .. Quantitative Transcriptomes Analysis of Wild Type and ompR mutant of K. pneumoniae ATCC43816 .

Purpose: This study aims to compare the transcriptome profiling (RNA-seq) of ompR mutant to that of WT and to evaluate the transcrptional regulation of OmpR in K. pneumoniae. Overall design: mRNA profiles of K. pneumoniae ATCC43816 wild-type (WT) and ompR knockout mutant were generated by deep sequencing, in duplicate, using Illumina Illumina NovaSeq 6000. The sequence reads that passed quality filters were analyzed at the transcript isoform level with RSEM using the Expectation Maximization (EM) algorithm. TPM method is used to calculated expression level.