Effect of depletion of id2b on gene expression during zebrafish heart development

Purpose: To investigate other possible phenotype of id2b mutant and the mechanism of how id2b fuctions in heart morphogenesis. Methods: Approximate 1000 hearts dissected from 120 hpf id2b+/+; Tg(myl7:mCherry) and id2b-/-; Tg(myl7:mCherry) embryos were collected for RNA sequencing. Results: Enrichment analysis of DEGs demonstrated that the top-ranked anatomical structures affected by id2b deletion included the heart valve, the compact layer of ventricle, and the atrioventricular canal, id2b inactivation also impacted phenotypes such as cardiac muscle contraction and heart contraction. Conclusions: Zebrafish id2b loss of fuction leads to abnormal heart valve and heart contraction defect. Besides, id2b regulates heart contraction through controlling nrg1 synthesis. 120 hpf id2b+/+; Tg(myl7:mCherry) and id2b-/-; Tg(myl7:mCherry) zebrafish hearts were collected for RNA-seq.