Effects of Feed Enriched with Freshwater Fish Oil on the Nutritional Profile and Growth Performance of Asian Redtail Catfish (Hemibagrus nemurus) Fry

This study was conducted to determine the effect of feed enriched with freshwater fish oil (FFO) on the composition of fatty acids, amino acids, growth performance, survival, and seed yield of Asian red-tailed catfish (Hemibagrus nemurus). A total of 1,125 seeds (1.5 mg, 5 mm long) were divided into five groups, each with three replications. Maintenance was carried out in 15 aquariums (15 L of water, 75 seeds per tank) for 40 days. The feed was enriched with 0%, 8%, 10%, 12%, 14% FFO, and the seeds were fed as much as they could. The results showed that feed enrichment with FFO significantly affected the composition of fatty acids, amino acids, growth performance, and survival (p<0.05). Proximate analysis showed differences in crude protein and fat in feed, with total n-3 content increasing from 0.42% to 0.72%, and n-9 increasing from 21.96% to 27.3%. Meanwhile, n-6 decreased from 8.73% to 5.17%, and unsaturated fatty acids increased from 52.88% to 64.14%. The EPA content increased from 1.33% to 1.67% in the 14% FFO diet. The total amino acid content increased from 43.69% to 61.67%, with methionine, tyrosine, and leucine as the most abundant essential amino acids. The final body weight and survival rate of fry fed with 10% FFO were 875.27 mg and 82.22%, respectively. In conclusion, among the treatments involving feed enrichment with FFO, the feed enriched with 10% FFO resulted in the highest growth and survival rates of Hemibagrus nemurus fry.

本研究旨在探究添加淡水鱼油（freshwater fish oil, FFO）的饲料对亚洲红尾鲶（Hemibagrus nemurus）的脂肪酸组成、氨基酸组成、生长性能、存活率及种苗产量的影响。实验共计选用1125尾初始体重1.5mg、体长5mm的种苗，将其分为5组，每组设置3个生物学重复。实验在15个水体体积为15L的水族箱中开展，每箱放养75尾种苗，饲养周期为40天。实验饲料分别添加0%、8%、10%、12%、14%的FFO，投喂量以种苗饱食为原则。研究结果表明，添加FFO的饲料对受试鲶鱼的脂肪酸组成、氨基酸组成、生长性能及存活率均具有显著影响（p<0.05）。常规营养成分分析显示，饲料中的粗蛋白与粗脂肪含量存在差异，其中总n-3多不饱和脂肪酸含量从0.42%提升至0.72%，n-9多不饱和脂肪酸从21.96%升至27.3%；与此同时，n-6多不饱和脂肪酸从8.73%降至5.17%，总不饱和脂肪酸含量从52.88%提升至64.14%。在添加14%FFO的饲料组中，二十碳五烯酸（eicosapentaenoic acid, EPA）含量从1.33%增至1.67%。总氨基酸含量从43.69%提升至61.67%，其中蛋氨酸、酪氨酸与亮氨酸为含量最高的必需氨基酸。投喂10%FFO饲料的种苗，其终末体重为875.27mg，存活率为82.22%。综上，在本次FFO饲料添加梯度处理中，添加10%FFO的饲料可使亚洲红尾鲶种苗获得最优的生长性能与存活率。