Quantitative Proteomic Analysis of MARC-145 Cells Infected with a Mexican Porcine Reproductive and Respiratory Syndrome Virus Strain Using a Label-Free Based DIA approach

Porcine
reproductive and respiratory syndrome (PRRS) is an infectious
disease characterized by severe reproductive failure in sows, acute
respiratory disorders in growing pigs, and high mortality in piglets.
The causative agent of this syndrome is the PRRS virus (PRRSV), an
RNA virus belonging to the Arteriviridae family. To date, several
quantitative approaches of proteomics have been applied to analyze
the gene expression profiles during PRRSV infection in PAMs and MARC-145
cells, and few proteins have been consistent among independent studies,
probably due to the differences in the levels of virulence of different
PRRSV strains used and/or due to analytical conditions. In this study,
total proteins isolated from noninfected and infected MARC-145 cells
with a Mexican PRRSV strain were relatively quantified using label-free
based DIA approach in combination with ion-mobility separation. As
a result, 1456 quantified proteins were found to be shared between
the control and infected samples. Afterward, these proteins were filtered,
and 699 of them were considered without change. Also, 17 proteins
were up-regulated and 19 proteins were down-regulated during the PRSSV
infection. Bioinformatic analysis revealed that many of the differentially
expressed proteins are involved in processes like antigen processing,
presentation of antigens, response to viruses, response to IFNs, and
innate immune response, among others. The present work is the first
one which provides a detailed proteomic analysis through label-free
based DIA approach in MARC-145 cells during the infection with a Mexican
PRRSV strain.