Ultrasound-directed emergence of pluripotent-like spheroids from human fibroblasts

We have developed a gene- and chemical-free method of generating pluripotency from HDF cell, we called pluripotent-like spheroids yielded by ultrasonic stimulus (Physics). Physics were very similar to embryoid bodies (EB) in terms of cluster formation, pluripotency with three germ layer markers, and multi-lineage differentiation potential. To microarrys assay, cells were cultured period to 6 or 40 days with hESC medium after ultrasound stimulus.