Characterization of Vaginal Microbiota in Third Trimester Premature Rupture of Membranes Patients through 16S rDNA Sequencing

In China, premature rupture of membranes (PROM) count as a major pregnancy complication in China and usually result into adverse pregnancy outcomes. We analysed the vagina microbiome composition using 16S rDNA V3-V4 amplicon sequencing technology, in this prospective study of 441 women in their third trimester pregnancy. We first divided all subjects into PROM and HC (healthy control) groups, in order to investigate the correlation of vagina microbiome compo-sition and the development of PROM.