Effect of kirenol on oxidative stress injury in rats with cerebral ischemia/reperfusion injury based on PI3K/AKT/FoxO3a signaling pathway

AimTo investigate the effect of kirenol on oxidative stress injury in rats with cerebral ischemia/reperfusion injury based on PI3K/AKT/FoxO3a signaling pathway.MethodsThread embolism was used to establish rat model of cerebral ischemia/reperfusion injury by middle cerebral artery occlusion. Then the rats were divided into the sham group, model group (MCAO group), kirenol low-dose, medium-dose and high-dose groups (1.25, 2.5, 5 mg·kg-1), and nimodipine group (NMDP). The kirenol groups were given drug intervention once a day for seven days. The mNSS Scores and Corner test were used to evaluate the effect of kirenol on neural function in rats. Nissl staining and TUNEL staining were used to observe the ischemic cerebral cortex tissue in rats. The kit was employed to detect kirenol effects on the levels of SOD, GSH, MDA and CAT. DCFH-DA probe was used to detect the ROS level in ischemic cerebral cortex of rats. The protein expressions of cleaved caspase-3, caspase-3, Bax, Bcl-2, NeuN, SOD2, PI3K, AKT, p-AKT and p-FoxO3a were detected by Western blot.ResultsCompared with MCAO group, kirenol intervention significantly reduced mNSS score and corner turning percentage (P P P P ConclusionsKirenol may inhibit apoptosis and reduce oxidative stress injury in MCAO rats by regulating PI3K/AKT/FoxO3a signaling pathway, thus playing a neuroprotective role.