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Data from: Known knowns, known unknowns, unknown unknowns and unknown knowns in DNA barcoding: a comment on Dowton et al.

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DataONE2014-08-07 更新2024-06-27 收录
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In a recent commentary, Dowton et al. (2014) propose a framework for "next-generation" DNA barcoding, whereby multi-locus datasets are coupled with coalescent-based species delimitation methods to make specimen identifications. They claim single-locus DNA barcoding is outdated, and a multilocus approach superior, with their assertions supported by an analysis of 33 species of Sarcophaga flesh flies. Here, we reanalyse their data and show that a standard DNA barcode analysis is in fact capable of identifying 99.8% (all but one) of their Sarcophaga specimens, and that their conclusions misrepresent their data. We also discuss the benefits and drawbacks to their vision of "next-gen" barcoding.
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2014-08-07
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