The main driver of soil organic carbon differs greatly between topsoil and subsoil in a grazing steppe

1. Soil organic carbon (SOC) dynamics is regulated by a complex interplay of factors such as climate and potential anthropogenic activities. Livestocks play a key role in regulating the C cycle in grasslands. However, the interrelationship between SOC and these drivers remains unclear at different soil layers, and their potential relationships network have rarely been quantitatively assessed. 2. Here, we completed a six-year manipulation experiment of grazing exclusion (no grazing: NG) and increasing grazing intensity (light grazing: LG, medium grazing: MG, heavy grazing: HG). We measurements of light fraction organic carbon (LFOC) and heavy fraction organic carbon (HFOC) in 12 plots along grazing intensity in three soil layers (topsoil: 0-10 cm, mid-soil: 10-30 cm, subsoil: 30-50 cm) to assess their underlying controls. 3. Grazing significantly reduced SOC of the soil profile, but with significant depth and time dependencies. (1) SOC and SOC stability of the topsoil is primarily regula...