RNAseq for Young Old mouse liver hepatocytes with and without high fat diet. RNAseq for Young Old mouse liver hepatocytes with and without high fat diet

Our Goal was to determine how hepatocytes of young and old mice differentially respond to 9-weeks of high fat diet vs normal chow. Overall design: Upon receipt mice were rested for at least 2-weeks prior to initiation of experiment in which mice were maintained on either High fat diet (58y1, TestDiet) or normal CHOW (Teklad global 18% protein) for 9-weeks prior to euthanasia and hepatocyte isolation.