axiom 820K array Triticum aestivum

The introgression of novel genes from progenitors and related species has been widely employed to improve the agronomic characteristics of hexaploid wheat. This approach, however, has been hampered by the lack of markers that can be used to track introduced chromosome segments. Here, we describe the identification of a large number of single nucleotide polymorphism-based markers that can be used to genotype hexaploid wheat and to identify and track introgression from a variety of sources representing its secondary and tertiary gene pools. We have validated our markers using a high density Axiom? genotyping array. Use of the ultra-high density platform to genotype a range of diploid, tetraploid and hexaploid wheat lines and wheat relatives has confirmed the utility of the markers across a range of species.