Nanotiming: single-molecule based, telomere-to-telomere DNA replication timing profiling by nanopore sequencing

Dataset for the manuscript "Nanotiming: telomere-to-telomere DNA replication timing profiling by nanopore sequencing" by Theulot et al ,2024 (https://doi.org/10.1038/s41467-024-55520-3) related to the github repository (https://github.com/LacroixLaurent/NanoTiming)

WT_rep3.tar.gz contains fast5 file from an experiment where yeast BT1 strain was grown for one doubling time with 5µM BrdU then DNA was sequenced on R9.4.1 ONT flowcell

mod_mapping.bam contains the bam file resulting from the BrdU base calling with megalodon (v2.2.9) using our BT1 reference genome and our BrdU aware model for base-calling

WT_rep3_nanoT.bed.gz contains the reads coordinates from the mod_mappings file

WT_rep3_nanoT_alldata.rds contains the BrdU profiles for each reads of the mod_mappings file, with the BrdU signal binned in 1kb non overlaping windows

WT_rep3_nanoT.rds contains the genomic BrdU signal profiles by 1kb non overlaping windows

TeloLengthDataNanoT.rds contains all the telomeric sequences extracted from the experiments reported in the Figure 4 and S19 to S23 of the manuscript with the associated filtering information and nanotiming signal.