Novel amphiphilic polyvinylpyrrolidone (PVP) functionalized silicone particles as carrier for low cost lipase immobilization

The high catalytic activity, specificity and stability of immobilization lipase have been drawn a great interests. How to reduce the cost of support materials has always been a hot topic in this field. Herein, for the development of low-cost immobilized lipase, we demonstrate an amphiphilic polyvinylpyrrolidone (PVP) grafted on silicone particles (SP) surface materials (SP-PVP) with rational design based on interfacial activation and solution polymerization. Meanwhile, the hydrophilic pristine SP and hydrophobic polystyrene-corded silicone particles (SP-Pst) were also prepared for lipase immobilization. The SP-PVP were characterized by X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy, Fourier transforms infrared spectroscopy and thermogravimetry. Our results indicated that the lipase loading amount on the SP-PVP composites was about 215 mg of protein per g. In the activity assay, the immobilized lipase SP-PVP@CRL exhibited higher catalysis activity, bett...