Gut microbiome of mice treated with antibiotics and fed different diets Targeted loci

The present study was undertaken to examine the role of diet in affecting the intestinal microbiome; particularly in the context of antibiotic-induced perturbations in an intestinal ecosystem. We sought to perform quantitative analyses to previously reported studies that relied on culture methods or qPCR to quantify perturbation of the16S gene in studies of the microbiome. The fecal microbiomes of mice treated with a cocktail of broad-spectrum antibiotics while being fed two different laboratory chows- including a partially defined mouse chow that is commonly used for nutritional studies - were analyzed by next generation sequencing of the V3 regions of the 16S rRNA gene.