Annotating Low Abundance and Transient RNAs in Yeast using Ultra High-throughput Sequencing

Annotating Low Abundance and Transient RNAs in Yeast using Tiling Microarrays and Ultra High-throughput Sequencing Reveals New Transcripts that are not Conserved Across Closely Related Yeast Species A complete description of the transcriptome of an organism is crucial for a comprehensive understanding of how it functions, how its transcriptional networks are controlled, and may provide insights into the organism__s evolution利用瓦片微阵列（Tiling Microarrays）与超高通量测序（Ultra High-throughput Sequencing）对酵母中的低丰度瞬时RNA进行注释，揭示了近缘酵母物种间不保守的新型转录本。完整解析生物体的转录组，对于全面理解其功能运作、转录调控网络的调控机制至关重要，同时也可为该生物体的进化研究提供新的洞见。