File S1 - Genome-Wide Sequencing of Cellular microRNAs Identifies a Combinatorial Expression Signature Diagnostic of Sepsis

File S1 contains 4 parts: supporting information S1, Figure S1, Table S1 and Figure S2. Figure S1. Neutrophil CD64 expression in different groups. (A) Each point represents the expression of CD64 on neutrophils for an individual patient expressed as MFI (Mean Fluorescent Intensity). The bar represents the geometric mean of each group. Kruskal-Wallis ANOVA test was applied. *** significant at p<0.001. (B) A representative FACS dot plot showing neutrophils gated by CD66b and high side scatter. (C)&(D) show histogram of isotype FITC expression and CD64 expression of two ICU patients representing high (black line) or low (grey solid) expression of CD64. (E) ROC Curves demonstrate the diagnostic utility of CD64 in comparison with WBC, CRP and temperature for sepsis in ICU. (F) The correlations of neutrophil CD64 with APACHE II score; (G): The correlations of neutrophil CD64 with SOFA score. Figure S2. miR-146a and miR-223 expression level in different groups (UK cohort). Each bar represents the expression of miR-146a (left) or miR-223 (right) in healthy volunteers, Sepsis patients or SIRS patients; Kruskal-Wallis ANOVA test was applied. *** significant at p<0.01; *** significant at p<0.001.
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