Supplementary Material for: Oncogenic Properties of Candidate Oncogenes in Chromosome Region 17p11.2p12 in Human Osteosarcoma

Osteosarcomas are primary tumors of bone that most often develop in adolescents. They are characterized by complex genomic changes including amplifications, deletions, and translocations. The chromosome region 17p11.2p12 is frequently amplified in human high grade osteosarcomas (25% of cases), suggesting the presence of one or more oncogenes. In previous studies, we identified 9 candidate oncogenes in this region <i>(GID4</i>,<i> ARGHAP44</i>,<i> LRRC75A-AS1</i>, <i>TOP3A</i>,<i> COPS3</i>,<i> SHMT1</i>,<i> PRPSAP2</i>,<i> PMP22</i>, and<i> RASD1)</i>. The aim of the present study was to determine their oncogenic properties. Therefore, we generated osteosarcoma cell lines overexpressing these genes, except for <i>LRRC75A-AS1</i> and <i>PRPSAP2</i>, and subjected these to functional oncogenic assays. We found that <i>TOP3A</i>, <i>SHMT1</i>, and <i>RASD1</i> overexpression provided increased proliferation and that <i>ARGHAP44</i>, <i>COPS3</i>, and <i>PMP22</i> overexpression had a stimulatory effect on migration and invasion of the cells. <i>COPS3</i> and <i>PMP22</i> overexpression additionally improved the ability of the cells to form new colonies. No oncogenic effect could be demonstrated for <i>GID4</i> overexpression. We conclude that the concerted amplification-mediated overexpression of these genes in 17p11.2p12 may contribute to the oncogenic process in malignant osteosarcoma.