The Darwin Tree of Life (DToL) Nematostella vectensis reference genome jaNemVect1.1 with the addition of the mCherry transgene used in this study. GTF transcriptome annotation file is included.

We performed bulk RNA transcriptome analysis of a Nematostella vectensis transgenic reporter line expressing mCherry under the regulation of the RIG-I-like receptor (RLRb). We sorted gastrula stage embryo derived cells treated from zygotes injected with the viral mimic poly(I:C) using fluorescence-activated cell sorting (FACS). RNA was extracted from mCherry positive and negative cells and used for constructing libraries for RNA sequencing.Additionally, two-day-old wild-type animals were dissociated and subjected to intracellular immunofluorescence. RLRb cells with high (approximately 15%) and low expression levels were isolated using FACS and processed for RNA sequencing.