4T1 PBS vs ADV gene.TPM.matrix.annot.xls

For studies of orthotopically grown breast tumors, BALB/c mice were injected into the third mammary fat pad with 2.5×105 4T1 cells suspended in 100 μl of HBSS. When the tumors reached approximately 50–100 mm3, the mice were randomly grouped and intratumorally treated with an ADV dose of 5×107 pfu. The first administration was recorded as the first day, with injections every other day for a total of three times. The survival and tumor size of the animals were observed every other day. The tumor volume was calculated as 0.5 × length × width2. Three to five days after the last injection of the virus, the mice were sacrificed and the tumor tissues were collected for transcriptome sequencing.