five

Data supporting the publication: Structural remodelling and activation of the hdeAB and proVWX operons in response to pH and salt stress

收藏
DataCite Commons2026-03-10 更新2026-03-28 收录
下载链接:
https://data.4tu.nl/datasets/f75943dc-4404-4784-8445-ceee37f141ad/1
下载链接
链接失效反馈
官方服务:
资源简介:
This is data supporting the paper titled, "Structural remodelling and activation of the hdeAB and proVWX operons in response to pH and salt stress"it consists of:1.Raw data files of RT-qPCR experiments of the <em>hdeAB </em>and <em>proVWX </em>operon and their flanking genes in the MG1655 Δ<em>endA</em> (WT,NT331), MG1655 Δ<em>endA </em>Δ<em>stpA</em> (Δ<em>stpA, </em>NT633), MG1655 Δ<em>endA</em> Δ<em>hns</em> (Δ<em>hns, </em>NT794), MG1655 Δ<em>endA</em> Δ<em>hns</em> Δ<em>stpA</em> (Δ<em>hns</em> Δ<em>stpA, </em>XT3236), MG1655 Δ<em>endA</em> Δ<em>hns</em> Δ<em>stpA</em> Δ<em>rpoS</em> (Δ<em>hns</em> Δ<em>stpA</em> Δ<em>rpoS, </em>XT3238), MG1655 Δ<em>endA</em> Δ<em>rpoS </em>(<em>ΔrpoS</em>, XT3132)<em> </em>in different pH (pH6.6/2.0)or osmotic condition (NaCl 0.08 M/ 0.08 M to 0.3M).2.Raw data files of ChIP-qPCR experiments of the <em>hdeAB </em>and <em>proVWX </em>operon and its flanking genes in the MG1655 Δ<em>endA</em>; <em>hns</em>::<em>hns</em>-3XFLAG (NT796), MG1655 Δ<em>endA</em>; <em>stpA</em>::<em>stpA</em>-3XFLAG (NT797), MG1655 Δ<em>endA</em>;<em>hns</em>::<em>hns</em>-3XFLAG; <em>stpA::kanRccdB </em>(NT866), MG1655Δ<em>endA</em>; <em>stpA</em>::<em>stpA</em>-3XFLAG; <em>hns::kanRccdB </em>(NT867) at pH 6.6 and pH 2.0.3.Raw data files of 3C-qPCR experiments of the <em>hdeAB </em>and<em> proVWX </em>operon and their flanking region in different pH (pH6.6/2.0)or osmotic condition (NaCl 0.08 M/ 0.08 M to 0.3M). For hdeAB operon, we use the transcription start site fragment (fragment 10 in our study) as the anchor fragment in MG1655 Δ<em>endA</em> (WT,NT331), MG1655 Δ<em>endA </em>Δ<em>stpA</em> (Δ<em>stpA, </em>NT633), MG1655 Δ<em>endA</em> Δ<em>hns</em> (Δ<em>hns, </em>NT794), MG1655 Δ<em>endA</em> Δ<em>hns</em> Δ<em>stpA</em> (Δ<em>hns</em> Δ<em>stpA, </em>XT3236), MG1655 Δ<em>endA</em> Δ<em>hns</em> Δ<em>stpA</em> Δ<em>rpoS</em> (Δ<em>hns</em> Δ<em>stpA</em> Δ<em>rpoS, </em>XT3238), MG1655 Δ<em>endA</em> Δ<em>rpoS </em>(<em>ΔrpoS</em>, XT3132).4.Agarose gel images in this study. 5.All the raw data used for figure generation in RT-qPCR, 3C-qPCR, ChIP-qPCR, MST, and bridging assays.
提供机构:
4TU.ResearchData
创建时间:
2026-02-11
二维码
社区交流群
二维码
科研交流群
商业服务