Methamphetamine induces transcriptional changes in cultured HIV-infected mature monocytes that may contribute to HIV neuropathogenesis

We performed RNA sequencing to characterize the effects of methamphetamine on HIV-infected mature monocytes. In vitro cultured HIV-infected mature (CD14+ CD16+) monocytes were treated with and without methamphetamine for 6h. RNA was extracted, sequenced, and analyzed for differential gene expression. We compared gene expression profiles between in vitro cultured HIV infected mature monocytes treated with or without methamphetamine for 6 hours (n=3 independent biological samples).