Chlamydomonas strains used in this study and presence of discussed features.

Some of the tomograms from these strains were previously used with different image processing methods to analyze other axonemal complexes [30], [37], [51].aStrain provided by Raqual Bower and Mary E. Porter (University of Minnesota). The N-DRC defective pf2 mutant strain [36] was rescued with a GFP-tagged WT PF2 gene. The resultant strain, pf2-4::PF2-GFP, is structurally and phenotypically indistinguishable from WT.bStrain provided by Douglas Tritschler and Mary E. Porter (University of Minnesota). The N-DRC defective ida6 mutant strain [78] was rescued with a GFP-tagged WT IDA6 gene and the resulting ida6::IDA6-GFP strain has the same motility and structure as WT.cStrain provided by Douglas Tritschler and Mary E. Porter (University of Minnesota). The N-DRC defective ida6 mutant strain [78] was rescued with a SNAP-tagged WT IDA6 gene. The resultant strain, ida6::IDA6-SNAP, is structurally and phenotypically indistinguishable from WT.