Reconstruction of human alveoli in vivo using fiber-shaped expansion system of iPS cell-derived lung progenitor cells

We transplanted human iPS cell-derived lung progenitors into the mouse lung. In addition, human iPS cell-derived lung progenitors were expanded in the cell-encapsulated alginate microfibers. In each case, the lung progenitors were differentiated into respiratory epithelial cells in vitro and in vivo, respectively. Lung progenitors were induced from human iPS cells and the engrafted cells were collected eight weeks after transplantation of lung progenitors into the mouse lung. On the other hand, human iPS cell-derived lung progenitors were expanded in the cell-encapsulated alginate microfibers (three-dimensional culture) for four weeks and differentiated into alveolar and airway epithelial cells for additional two weeks. The transcriptomes of those cells were obtained based on paired-end reads (100-bp) with Illumina Novaseq 6000.