Gene expression analysis in proximal and distal colons of Lcn2-/- IL10-/- double knock-out mice

Lcn2 is involved in host defense against pathogens, but the function in intestinal mucosal immunity and inflammation remains largely unknown. Genetic ablation of Lcn2 results in early-onset colitis and spontaneous emergence of right-sided colonic tumors in the setting of IL-10 deficiency (Lcn2-/-;IL10-/- mice). To address whether inflammation or other mechanisms drives the site-specific tumor locations gene expression analyses in proximal versus distal colons of Lcn2-/- IL10-/- mice were performed. Differential expression between distal colon versus cecum and proximal colon samples were analyses using Affymetrix MoGene 2.0 ST arrays on formalin-fixed, paraffin-embedded tissue sections of Lcn2-/-; IL10-/-mice.脂质运载蛋白2（Lcn2）参与宿主对抗病原体的防御过程，但其在肠黏膜免疫与炎症中的功能仍未被充分阐明。当小鼠存在白细胞介素10（IL-10）缺陷时，敲除Lcn2可导致其出现早发性结肠炎，并自发形成右侧结肠肿瘤，此即Lcn2-/-;IL10-/-小鼠模型。为明确究竟是炎症还是其他机制驱动了肿瘤的部位特异性分布，研究人员对该模型小鼠的近端结肠与远端结肠开展了基因表达分析。本次分析采用Affymetrix MoGene 2.0 ST基因芯片，对Lcn2-/-;IL10-/-小鼠的福尔马林固定石蜡包埋组织切片（分别取自盲肠、远端结肠与近端结肠）进行了差异表达分析。