Single cell RNA sequencing reveals myeloid-biased hematopoiesis and pro-inflammatory state in TLR9-/- bone marrow

The TLR9-/- mice had more myeloid cells and progenitors and fewer B cells in the bone marrow. Activity of Fos and Cebpb, important mediator of myelopoiesis, in the cluster of HSPCs were increased. PU.1 and Fos, critical transcription factors mediating osteoclastogenesis, were upregulated in monocyte progenitors. In addition to myeloid biased hematopoiesis in TLR9-/- mice, there were upregulated levels of inflammatory cytokines in the TLR9-/- bone marrow. Bone marrow mononuclear cells from 2 wildtypes and 2 TLR9-/- mice were profiled in two independent experiments (each experiment included samples from 1 wildtype and 1 TLR9-/- mice).