mRNA and lncRNA expression profiling analysis in A549 cells treated with DMSO, RSL3 alone, or co-treated with Fer-1

Ferroptosis is a new type of programmed cell death induced by iron-dependent lipid peroxidation that has been linked to several malignancies, including non-small cell lung cancer (NSCLC). Finding ferroptosis-associated genes is extremely helpful for guiding and improving ferroptosis-based anti-tumor therapy. To explore ferroptosis-associated genes, we treated A549 cells with DMSO, RSL3 alone, or co-treated with Fer-1 for 24h. Lung cancer-associated transcript 1 (LUCAT1) was identified as a novel ferroptosis suppressor via RNA-seq analysis. Overall design: To investigate the mRNAs and lncRNAs associated with ferroptosis, we treated A549 cells with DMSO, RSL3 (2µM) alone, or co-treated with Fer-1 (5µM) for 24h. We then performed gene expression profiling analysis using data from RNA-seq.