RNA sequencing determines nuclear-accumulated Cav3.2iPA on transcriptional regulation of human iPSC-derived sensory neurons

By targeting the intrinsically disordered regions (IDR) of calcium channel subtype 3.2 (Cav3.2), we discovered selective T-type/Cav3.2 peptide inhibitors (Cav3.2iPAs) for AAV-mediated peripheral sensory neuron-specific analgesia. We found that Cav3.2iPAs are highly accumulated in the nuclei after expression in human induced pluripotent stem cells-derived sensory neurons (hiPSC-SNs). This study aims to determine whether nuclear-accumulated Cav3.2iPAs disturb the transcriptional regulation of hiPSC-SNs which becomes a safety concern. RNA sequencing compares the transcriptomes in hiPSC-SNs-expressing Cav3.2iPA and hiPSC-SN-expressing Cav3.2NP (control)