Dual loss of Rnf8 and p53 leads to increased aneuploidy and genomic instability.

Splenocytes isolated from 6-week old Rnf8−/−p53−/− mice and control littermates were activated with LPS for 24 hours and then either left untreated (UT) or irradiated (2 Gy). 24 hours later metaphase spreads were prepared. Results shown are pooled from analyses of metaphase spreads from activated B-cells of at least two different mice per genotype. Second row (bold) - percentage data shown. Fusions involve dicentric chromosomes, sister chromatid fusion, ring chromosomes and Robertsonian fusion like configurations.