The plasma proteins from human multiple myeloma was enriched by lectin AANL6 and identified by LC-MS/MS

Lectin is widely used to enrich glycoconjugates carrying glycans and to identify the abnormal glycosylated proteins in tumor with mass spectrometry, which is a useful tool for cancer diagnosis and prognosis monitoring. In our previously reported, we identified a novel lectin AANL from Agrocybe aegerita that has a higher affinity than other lectins when binding with GlcNAc, and the lectin AANL has been used for early diagnosis of non-small cell lung cancer. Subsequently, AANL6, a mutant of AANL, is cloned and purified, and has higher affinity to terminal GlcNAc than AANL. Therefore, in this study, we used the lectin AANL6 for enrichment of glycosylated peoteins from MM, and identified with quantitative proteomics, in order to find a potential sensitive biomarker for MM diagnosis.