Miguel Vicente-Manzanares, Margaret A. Koach, Leanna Whitmore, Marcelo L. Lamers (2011) CIL:12410, Cricetulus griseus, epithelial cell. CIL. Dataset

Localization and intracellular motility of myosin IIA (MIIA) in protrusive regions of migrating CHO.K1 cells. Arrowheads point to locations where representative MIIA filaments form. This Video corresponds to Fig. 6 A and video 3 in JCB 183:543-554 , 2008. Images of GFP-MIIA were collected every 4 s using TIRF microscopy. Images were acquired in an inverted microscope (model IX70;Olympus) with 488 nm excitation. A dichroic mirror (HQ485/30) was used for GFP-labeled cells. Images were acquired with a charge-coupled device camera (Retiga Exi; Qimaging) and analyzed using MetaMorph software (MDS Analytical Technologies). 10 frames/s shown.

本研究探讨了肌球蛋白IIA（MIIA）在迁移性CHO.K1细胞突出区域中的定位及细胞内运动。箭头所指为典型MIIA丝状结构形成的位置。本视频对应于JCB 183:543-554（2008年）第6图A和视频3。利用TIRF显微镜每隔4秒采集GFP-MIIA图像。图像采集于倒置显微镜（型号IX70；Olympus）下，激发波长为488 nm。使用双色镜（HQ485/30）对GFP标记的细胞进行成像。图像通过电荷耦合器件相机（Retiga Exi；Qimaging）采集，并利用MetaMorph软件（MDS Analytical Technologies）进行分析。展示帧率为每秒10帧。