Data for: Assessing above and belowground recovery from ammonium sulphate addition and wildfire in a lowland heath: mycorrhizal fungi as potential indicators.

Atmospheric pollution containing soil-nitrifying ammonium sulphate ((NH4)2SO4) affects semi-natural ecosystems worldwide. Long-term additions of (NH4)2SO4 to nitrogen(N)-limited habitats, including heathlands, increase climate stress affecting recovery from wildfires. Although heathland vegetation largely depends on ericoid mycorrhizal fungi (ErM) to access soil N, we lack a detailed understanding of how prolonged exposure to (NH4)2SO4 may alter ErM community composition and host plants’ reliance on fungal partners following wildfire and affect recovery. Simulation of atmospheric pollution ((NH4)2SO4) occurred bi-weekly for 5 years after a 2006 wildfire in a UK heathland. Ten years after treatments ceased, we measured vegetation structure, lichen and lichen photobiont composition, soil characteristics, ErM colonisation, ErM diversity in roots and soil, and assessed ErM potential as novel recovery indicators. Heather height and density, and moss groundcover, were greater in N-enriched pl..., Total soil DNA was extracted in duplicates from 0.25 g of soil from each plot (two extractions per plot, n = 24, DNeasy PowerSoil Kit). The DNA extractions were processed by Macrogen (Seoul, South Korea) for library preparation and Illumina sequencing with the primer set ITS86F and ITS4 (White et al. 1990; Turenne et al. 1999) targeting the ITS2 region., , # Data for: Assessing above and belowground recovery from ammonium sulphate addition and wildfire in a lowland heath: mycorrhizal fungi as potential indicators

The dataset contains raw ITS2 reads obtained within the study \"Assessing above and belowground recovery from ammonium sulphate addition and wildfire in a lowland heath: mycorrhizal fungi as potential indicators\". The dataset was produced to assess the composition of soil fungal communities in a dry heathland ten years after cessation of ammonium sulphate treatments.

## Description of the data and file structure

Reads in this dataset are raw ITS2 obtained with the primer pair ITS86F and ITS4 and sequenced with Illumina technology.

## Sharing/Access information

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* DOI of the article once published in Restoration Ecology will be made availabl...