Characterization of rhizosphere and endophytic bacterial communities from roots nodules and roots of Vigna radiata by metagenomic analysis. root metagenome

Previous studies have focussed on presence of non rhizobial endophytes in root nodules of Vigna radiata. However, detailed knowledge concerning the bacterial community associated with V. radiata is still limited. Therefore, this study addressed two fundamental questions: (1) How does the bacterial community structure differ between the rhizosphere and different organs in V. radiata and (2) Do different habitats, such as rhizosphere and different tissues, have the potential to influence the structure of bacterial communities? In order to address these questions, the bacterial community structure and diversity in the rhizosphere, as well as in root nodules and roots of V. radiata were analyzed by amplifying V3 region of 16S rRNA gene.