Analysis of Pol II position in asynchronous, G1- or G2/M-arrested cells

We sequenced RNA Pol II Flag pulldowns (NET-seq) from five samples of W303 bar1D (asynchronous, alpha-Factor arrested, nocodazole arrested or transformed with a pGAL1 multicopy plasmid carrying either no ORF or the SEN1 ORF)我们对5株W303 bar1Δ（W303缺失bar1基因）酿酒酵母菌株样本的RNA聚合酶II（RNA Polymerase II，RNA Pol II）Flag标签下拉纯化产物进行了新生延伸转录组测序（Native Elongation Transcriptome Sequencing，NET-seq）。该5组样本分别为：异步培养菌株、α因子阻滞菌株、诺考达唑阻滞菌株，以及分别转化有携带空载体（无开放阅读框）的pGAL1多拷贝质粒、携带SEN1开放阅读框（Open Reading Frame，ORF）的pGAL1多拷贝质粒的转化菌株。