Scalable and cost-efficient custom gene library assembly from oligopools

This dataset contains all next-generation sequencing (NGS) data generated
to evaluate the OMEGA (Oligo-based Multiplexed Efficient Gene Assembly)
platform for multiplexed gene library construction. It includes PacBio
HiFi long-read sequencing (BAM with index and metadata), Illumina
paired-end sequencing (FASTQ), and Oxford Nanopore long-read sequencing
(FASTQ) across assembly validation (Rubisco and Cas9 libraries),
amplicon-based quality control (PCR and JSCAN), and functional screening
of GFP variant libraries. Data are organized by sequencing platform and
experiment type, including replicate-level presort libraries and
fluorescence-activated cell sorting (FACS) populations spanning multiple
fluorescence bins and negative controls. These files enable reconstruction
of full-length variants, quantification of assembly accuracy and
uniformity, and analysis of sequence–function relationships. All data are
standard DNA sequencing formats compatible with common open-source tools
and are suitable for benchmarking gene assembly methods, developing
analysis pipelines, and training machine learning models. All sequences
are synthetic or non-pathogenic research constructs; no human or clinical
data are included, and there are no ethical or legal restrictions on
reuse.