Selection of binders from the αRep libraries.

aThe libraries indicated were used for the selections against the corresponding targets.bThe phage-ELISA results are indicated as the number of clones giving a positive signal versus the number of clones tested. A Clone was scored as positive if its measured signal/noise ratio was greater than five.cThe sequences were determined among the phage-ELISA positive clones. For each target, the number of distinct sequences is indicated over the total number of sequences determined.dThe soluble expression of phage-ELISA positive clones was probed using CoFi blot or Western blot experiments after liquid expression cultures. Reported ratios indicate the number soluble proteins over the number of clones tested.eThe properties of the clones used for further characterization were determined by ITC, DSC and/or SEC as described below. The number of internal repeats for each binder is indicated in parentheses.