We firstly investigated the characteristics of soil archaea and its relationship to soil properties under different level of available P enrichment greenhouse vegetable soil.

The greenhouse vegetable field is a special ecosystem with characteristics of heavy anthropogenic disturbance and excess phosphorus (P) accumulation in soil. Thus soil microbial community structure and activity expression must have its special features which are quietly different from other natural ecosystems. We firstly investigated the characteristics of soil archaea and its relationship to soil properties under different level of available P enrichment greenhouse vegetable soil. The archaea structure was analyzed through high throughput sequencing using Miseq Illumina platform. 15 soils were sampled at 0-20 cm from different greenhouse fields of Beijing, and divide into groups based on the available P (Olsen-P) content (0-50 mg kg-1, 50-100 mg kg-1, 100-400 mg kg-1). We try to reveal the specificity of archaea structure and its relationship to soil P status by the analysis of bioinformatics and statistics. The clone library construction was employed using 16S rRNA gene as genetic marker. The cloning/sequencing of 16S rRNA genes showed that Euryarchaeota, Pacearchaeota, Woesearchaeota and Thaumarchaeotaare were the dominant phylums. Soil Olsen-P content had no significant influence on soil archaea community structure and diversity in the phylum level. In the genus level, however, the abundance of Methanomassiliicoccus, Pacearchaeota Incertae Sedis AR13 and Methanocorpusculum decreased with the increasing of Olsen-P content. While, the abundance of Nitrososphaera, Methanosarcina increased significantly. Even so, we should have more evidences to demonstrate that whether available P content is the direct factor for soil archaea community structure and activity.