Gut microbiota contribute to Red40-induced colitis

Previously we have shown that unfractionated mesenteric lymph node (mLN) CD4+ T cells obtained from R23FR mice in remission transfer colitis to Rag1-/- mice exposed to diet switches (R23FR to Rag transfer model)(Chen et al., 2018). This experiment is aim to define if specific bacterial strains contributed to Red 40 induced-colitis. These 16S rRNA amplicon sequence data were generated from DNA extracted from feces collected from Rag1-/- SPF mice after treated with vancomycin (500mg/L) and polymyxin B (100mg/L) for 5 weeks and GF Rag1-/- mice colonized with human stool library (library number #1001136) (Britton et al., 2019) (ex-GF mice).